Applied BioPhysics

🥳 research from the University at Albany CNSE

Led by Fernando Pesantez Torres, this study used ECIS to track how NIH 3T3 fibroblasts and bEnd3 endothelial cells respond to SMA nanoparticles in real time.

🧪 Negligible cytotoxicity at all doses
🛡️ High NP levels slightly slowed barrier formation
🔄 Medium change restored barrier function at low doses; high doses remained reduced, likely from NP attachment

The results underscore ECIS as a powerful platform for monitoring cell behavior and guiding the safe design of oxygen-sensitive nanomaterials for biomedical use.

📖 Read the full study: https://pubs.acs.org/doi/10.1021/acsabm.5c01443

Using Z-Theta, researchers tracked how Müller glial cell EVs affect RPE barrier function:

🔵Normoxic EVs (blue): Barrier stays stable ✅
🟡Hypoxic EVs (yellow): Barrier weakens quickly, TER drops over 72h ⚠️

💡 Label-free, real-time monitoring shows how hypoxic EVs compromise retinal integrity—critical for diseases like diabetic retinopathy.

📄Full paper: https://www.mdpi.com/2079-7737/14/8/1014 #

Insulin-regulated palmitoylation plays a crucial role in maintaining the integrity of lymphatic endothelial cell (LEC) barriers. Researchers at Saint Louis University used to continuously monitor TEER in real-time under incubation conditions, revealing how changes in palmitoylation profiles directly affect LEC permeability and stability.

🔗 Study: https://lnkd.in/gu9sdwqJ
ℹ️About ECIS: https://lnkd.in/gEqFH5Bt

🔎Did You Know? The first paper was published in 1984—the same year The Terminator warned us about Skynet and the Macintosh redefined computing.🍏🤖💻

Here’s to 40 more years of transforming science and pushing the boundaries of possibility! 🥳

https://www.researchgate.net/publication/16609706_Monitoring_fibroblast_behavior_with_an_applied_electric_field