Genomax Technologies

Human blood proteomics (Part 2): How?

Methods for human blood proteins profiling can generally be categorized into mass spectrometry-based and affinity-based approaches.

Mass spectrometry is the most commonly used approach in proteomics. Plasma proteomic workflow typically involves enzymatic digestion of circulating proteins into peptides for identification and quantification of proteins by pattern matching against database. Peptides are separated into fractions by chromatographic methods and sequentially injected into the MS instrument where peptides are scanned, selected, fragmented and detected.

Mass spectrometry provides unparalleled, unambiguous identification of specific peptides including the isoforms, but its application to plasma proteomics at population level remains prohibitive due to slow throughput and inability to quantify low abundance proteins without extensive upfront sample separation.

Affinity-based approaches use specific protein-binding detection reagents as probes for molecular protein analyses. While antibodies are the best-established binders used in classical immunoaffinity methods for protein analysis, various recombinant antibody mimetics were also discovered and/or developed, including scFv, VHH, DARpins, and aptamers.

For confident detection (and quantification) in different applications, paired reagents are preferred, where dual binding serves to increase specificity, sensitivity and signal-to-noise ratios in assays.

Classical immunoassays including ELISA, IHC, IF, flow cytometry and western blots typically have a "one binder, one sample" setup. Affinity-based proteomic profiling technologies have been expanded to high-throughput, multiplexed, miniaturized systems by integrating paired affinity reagents for high sensitivity and high specificity, offering the advantage of detecting proteins across a wide dynamic range while minimizing the amount of starting material required and the analysis time needed.

Stay tuned to know more about different high-plex, ultra-sensitive affinity-based proteomic profiling platforms!

📢 Mitochondrial Function Analysis Workshop 2.0 (Hybrid Workshop)

🔬 Interested in mastering mitochondrial function analysis in biomedical research?

Join our Mitochondrial Function Analysis Workshop 2.0 and discover how to analyze cellular metabolism using the Agilent Real-time Mitochondrial Analyzer

📊 In this workshop, participants will gain insights into how researchers measure:
* Oxygen Consumption Rate (OCR)
* Extracellular Acidification Rate (ECAR)
* ATP Production
* Mitochondrial Respiration
* Metabolic Phenotype of Cells

Participants will also be introduced to mitochondrial assays widely used in research areas such as:
🧬 Ageing & Cellular Metabolism
🧬 Immune Cell Metabolism
🧬 Cancer Metabolism
🧬 Mitochondrial Fuel Pathways

👩‍🎓👨‍🔬 Who should attend?
Students • Academics • Researchers • Scientists

👩‍🏫 Speakers
* Prof. Dr. Suzana Makpol (Biochemistry Department, UKM)
* Dr. Norwahidah Abdul Karim (Biochemistry Department, UKM)
* Charlie Chin Ding Sheng (Genomax Technologies)
* David Chung Tzu Yang (Genomax Technologies)

📅 Date: 19 – 20 May 2026
📍 Venue:
* Lecture Hall 1
* Department of Biochemistry Seminar Room
Preclinical Building, Faculty of Medicine, UKM

💰 Early Bird (Before 1 April 2026)
⚠️Seats are limited for face-to-face participants.
📌 Registration Deadline: 18 May 2026
🔗 Register Now:
https://forms.gle/8ovF4mGJPwXByFZC6

📞 Contact:
Dr Norwahidah Abdul Karim (03-91459555)
Ms Nur Haleeda Hakimi (03-91458689)

For enquiries:
📧 [email protected]

🧬 High-Plex. Spatial Precision. Zero Compromise.

The gap between the transcriptome and the proteome has officially been bridged. With Bruker’s GeoMx® 1,200-plex Discovery Proteome Atlas (DPA), true multi-omics discovery is now a spatial reality.

What sets this apart isn't just the plex—it’s the precision. You can now capture deep protein expression profiles for every single Region of Interest (ROI) you select on your tissue section.

Why the DPA Panel is the new standard:

• True Multi-omics: Pair the Whole Transcriptome Atlas (WTA) with 1,200+ protein targets on a single slide.

• IHC-Validated: Every target is powered by IHC-validated antibodies, giving you the gold-standard reliability your peer-reviewed data demands.

• Functional Depth: Go beyond expression with coverage for over 130 post-translational modifications (PTMs).

• Granular ROI Insight: Get 1,200-plex data tailored to your specific morphological regions—from the tumor microenvironment to specific cell niches.

Stop guessing what’s happening between RNA and protein. Start seeing it in high resolution.

Is your lab ready to unlock the 1,200-plex spatial era? 🚀

Reach out to us at [email protected] to learn more about DPA, and for the full antibody clone list.