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Interested in getting your sequencing project funded? Submit a 250-word proposal for a SMRT Grant, offered by Pacbio certified service provider GENEWIZ. The deadline is March 25, 2018.

PacBio PAG Sequencing Grant | GENEWIZ Enter by 03/25/18 for the PacBio Grant with GENEWIZ, Learn more and enter here>>

The Mexican salamander, or the axolotl were sequenced 110 million long reads (32× coverage, N50 read length 14.2 kb) using Pacific Biosciences instruments to avoid the read sampling bias that is often found when using other technologies and to span repeat-rich genomic regions that cause breaks in short-read assemblies.The long reads also allowed to overcome several assembly challenges, such as 18.6 Gb of repetitive sequences (65.6% of the contig assembly) and distinct long terminal repeat (LTR) retroelement classes and endogenous retroviruses with elements more than 10 kb long.

Small Creature, Massive Genome: Axolotl Offers Insights Into Regeneration - PacBio PacBio is the leader in long-read sequencing, providing high-quality genomes to advance scientific discovery. Learn how SMRT Sequencing powers genomic insights in humans, plants, animals and microbes.

One single genome is not enough to represent the huge amount of variation in genomes. Hence, Pacbio full-Length Isoform sequencing of samples from different environmental conditions, would enable accurate whole plant transcriptome analysis. This would help the community to achieve a greater understanding of the impacts of structural variation on plant genomes which will result in crop improvement.

When A Single Reference is Not Enough - PacBio Maize is amazingly diverse. A study comparing genome segments from two in**ed lines, for instance, revealed that half of the …

Scientists sequenced Utricularia gibba genome using SMRT Sequencing. The genome was previously sequenced with short-read technology, producing an 82 Mb assembly. With PacBio long-read technology, the team was able to significantly improve on the original assembly. The de novo genome project resulted in an assembly with a contig N50 of nearly 3.5 Mb. The total size was about 100 Mb, adding more than 18 Mb missed by the short-read assembly. Twenty-four contigs included telomeres achieved, with four of those representing complete chromosomes.

Carnivorous Plant Relies on Tandem Duplications for Adaptation - PacBio Scientists from the University at Buffalo, Nanyang Technological University, and other institutions published results from an effort to elucidate the …