MolE - Molecular Engineering Lab

Congrats to all co-authors: Andrea Arsiccio & Paolo Giorsello & Livio Marenco! (✍️RP) 👏👏

"Considerations on Protein Stability during Freezing and its Impact on the Freeze Drying Cycle: A Design Space Approach"

Freezing is widely used during the manufacturing process of protein-based therapeutics, but it may result in an undesired loss of biological activity. Many variables come into play during freezing that could adversely affect protein stability, creating a complex landscape of interrelated effects. In this article we show how mathematical models can guide the selection of the optimal freezing protocol, focusing on protein stability. Two opposite scenarios are identified, suggesting that the ice-water interface is the dominant cause of denaturation for proteins with high bulk stability, while the duration of the freezing process itself is the key parameter to be controlled for proteins that are susceptible to cold denaturation. Experimental data for lactate dehydrogenase and myoglobin as model proteins support the model results.

📌Anyone clicking on the following link before February 15, 2020, will be taken directly to the final version of our article on ScienceDirect, which they are welcome to read or download. No sign-up, registration or fees are required.

https://authors.elsevier.com/a/1aIVS55Jf1ZLc

MolE on Twitter “Congrats to all co-authors From our research group a new "Considerations on Protein Stability during Freezing and its Impact on the Freeze Drying Cycle: A Design Space Approach" https://t.co/gVhwBBReRp (QbD)”

Francesca Susa👨‍🎓ha partecipato all'evento .PoliTo
Il è stato promosso dal Politecnico di Torino per festeggiare i 160 anni.
https://www.festivaltecnologia.it/ è nato per esplorare la relazione tra tecnologia e società con un approccio umanistico e democratico.

Dario Casà👨‍🎓
Yuri Kitamukai👨‍🎓
Lorenzo La Cortiglia👨‍🎓
" Tra vent’anni non sarete delusi delle cose che avete fatto ma da quelle che non avete fatto. Allora levate l’ancora, abbandonate i porti sicuri, catturate il vento nelle vostre vele. Esplorate, sognate, scoprite. - Mark Twain"