GMA GROUP

In recent decades, the strains of pathogens that are resistant to the action of various drugs have become widespread worldwide. The resistance of microorganisms to the antibiotics can be natural or acquired, but forming the resistance in both cases is based on genetic factors.

There are widely spread and well known methicillin-resistant strains of Staphylococcus spp. (MRS – methicillinresistant Staphylococcus), including Staphylococcus aureus (MRSA – methicillin-resistant Staphylococcus aureus), coagulase-negative Staphylococcuses: S. epidermidis, S. saprophyticus and others (MRCoNS – methicillin-resistant coagulase-negative staphylococci).

Resistance to methicillin and oxacillin is caused by presence of penicillin binding protein (PBP2A – penicillin binding protein 2A) in the microorganism, which is coded by the mecA gene, transmitted as part of the mobile element ssc (staphylococcal cassette chromosome).

The MRS/MRSA Multiplex REAL-TIME PCR Detection Kit is intended for detection of Staphylococcus DNA and identification of mec A gene which is responsible for methicillin resistance.

Sample: urine; sputum; breast milk; cerebrospinal fluid; epithelial cell scrapes from urethra, cervical canal, posterior vaginal vault; oropharyngeal and nasal smears and washings; feaces; tracheae aspirate; swipes and washings from wound surface

DNA extraction: PREP-NA, PREP-GS, PREP-RAPID extraction kits